A furious Oprah Winfrey has fired off a message of warning to Rosie O’Donnell – no more gay talk about her or BFF Gayle King.

Oprah became upset when she learned Rosie was once again spouting the old rumors that Oprah and Gayle are gay lovers.

While promoting her new satellite radio show, acid-tongued Rosie happily stoked the embers of speculation on Howard Stern’s Oct. 26 radio show.

"I don’t know that she and Gayle are necessarily doing each other," Rosie quipped to Stern, "but I think they are the emotional equivalent of…."

"A gay couple," the shock jock finished.

That was all raucous Rosie needed to shift into high gear.

"When they did that road trip, that’s as gay as it gets," she responded, "and I don’t mean it to be an insult, either. I’m just saying, listen, if you ask me, that’s the couple."

After joining The View back in 2006, Rosie sparked controversy when she said the behavior of Oprah and her longtime best friend Gayle was "very typical of gay relationships."

When Oprah learned O’Donnell was again playing the gay card, she became enraged.

"Oprah exploded when she heard Rosie was again talking trash about her and Gayle," a close source told The ENQUIRER.

"Oprah was so furious she had a warning message sent to Rosie.

"She is making it very clear to her not to use her to promote her new radio show – and further, never again does she want to hear that Rosie is raising the gay thing about her and Gayle.

"Oprah has gone out of her way to say repeatedly that they are just good friends and are not gay."

Rosie came out of the closet as a lesbian in 2002, two months before her long-running syndicated TV talk show went off the air. She legally married longtime lover Kelli Carpenter, but the two have since split, as The ENQUIRER was the first to reveal.

Oprah is critical of Rosie because Rosie’s TV show began in 1996, but the so-called "Queen of Nice" took six years before telling the truth about her sexual orientation.

"Oprah said if she were gay, she would say so," said the source.

"She not only supports the gay community, but she also has many friends and employees who are gay.

"Oprah hopes that her words of warning get back to Rosie. Oprah snapped, ‘I don’t want to hear it any more!’"